Jorge U Carmona
1Universidad de Caldas, Colombia 2Universidad del Tolima, Colombia
Title: Histological and gene expression effects of platelet-rich gel supernatants in vitro system equine cartilage degeneration
Biography
Biography: Jorge U Carmona
Abstract
Introduction: Platelet-rich plasma (PRP) preparations are a common treatment in equine osteoarthritis (OA). However, there are controversies regarding the ideal concentration of platelets and leukocytes in these biological substances necessary to induce an adequate anti-inflammatory and anabolic response in articular cartilage.
Aims & Methods: The aims were to study the influence of leukocyte- and platelet-rich gel (L-PRG) and pure platelet-rich gel (P-PRG) supernatants on the histological changes of cartilage, the degree of chondrocyte apoptosis, the production of hyaluronan (HA) and the gene expression of nuclear factor kappa beta (NFkb), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), COL2A1 and cartilage oligomeric matrix protein (COMP) in normal cartilage explants (CEs) challenged with lipopolysaccharide (LPS).
Results: Overall, 25 % L-PRG supernatant (followed in order of importance by, 50% P-PRG, 25% P-PRG and 50% L-PRG) represented the substance with the most important anti-inflammatory and anabolic effect. 25% P-PRG supernatant presented important anabolic effects, but it induced a more severe chondrocyte apoptosis than the other evaluated substances.
Conclusions: 25% L-PRG supernatant presented the best therapeutic profile. Our results demonstrate that the biological variability of PRP preparations makes their application rather challenging. Additional in vivo research is necessary to know the effect of PRP preparations at different concentrations.